pDisplay-Insert
Forward Primer
Plasmid annealing = 0°C Target annealing = 0°C Length = 60
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XXXXXXTAXXXXAXXUXUXXXXXXXTAXXXXUXXXXTTXXXAXXCXATCXXXXXGXXXAX
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Reverse Primer
Plasmid annealing = 0°C Target annealing = 0°C Length = 60
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NAANNNNNNNTNNTNNNCNNNNNGATNGNNTNNNANNANNNNTANNNNNNNANANNTNNN
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New construct
ATGC = Original plasmid sequence. ATGC = Inserted sequence.
Plasmid annealing = 0°C Target annealing = 0°C Length = 60
Reverse Primer
Plasmid annealing = 0°C Target annealing = 0°C Length = 60
| 1° PCR Size | New Plasmid Size | Insert Sites | Insert Size |
| 2197bps | 4253bps |
|
2127bps |
| 2° PCR conditions | ||
| Extension Time | ng of insert | ng of plasmid |
| 1:24 mins | 527.3 | 25.5 |
New construct
ATGC = Original plasmid sequence. ATGC = Inserted sequence.
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Feb 06 2026
Plasmid markers and restriction enzymes:
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