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rf-cloning troubles

Topic	Replies	Views	Last post
2,881 Rf Cloning false positives by filipebaeta	1	7,074	2017-01-23 15:49:44 by Steve Bond
2,882 How to get insert:vector ratio in the 2' PCR? by minyoungfan33	1	7,511	2016-10-05 10:55:01 by Steve Bond
2,883 Relevance of DpnI treatment? by Alexander	4	23,771	2016-05-09 12:37:42 by CharlesPt
2,884 Amount of mega primer/plasmids second PCR. by Jan	5	17,478	2016-05-09 12:36:35 by CharlesPt
2,885 Problem with RF cloning by shrikant	1	7,549	2016-05-01 14:06:31 by Steve Bond
2,886 No colonies & no band on agarose gel after 2°PCR by afahrny	3	13,473	2015-12-21 11:24:24 by Steve Bond
2,887 to design an RF cloning project with the website by mandyscott	0	9,452	2015-12-21 06:00:15 by mandyscott
2,888 No band for products at all after primary PCR by bubunmicro@gmail.com	7	28,615	2015-06-30 20:14:40 by Steve Bond
2,889 suggestions for primary PCR by vithya	5	18,454	2015-05-10 14:22:04 by Steve Bond
2,890 RF-cloning to do mutagenesis? by erin.green	6	18,681	2015-02-18 17:16:07 by Steve Bond
2,891 Procedure after DpnI digestion by hongrulin	4	17,483	2015-02-18 17:15:33 by Steve Bond
2,892 DpnI by Sand	1	8,103	2015-01-30 16:15:24 by Steve Bond
2,893 Is phosphorilation needed? by Dungeonlord	18	56,942	2015-01-24 00:10:26 by Steve Bond
2,894 2nd PCR not working by ZacJ	8	81,142	2014-12-16 16:58:59 by ayidazagu
2,895 RFP + gene of interest RF cloning into vector in place of GFP ? by vsustar	2	31,339	2014-12-14 23:22:26 by LucilleHib
2,896 Problematic to say the least by pcr	7	26,445	2014-12-10 22:51:54 by Steve Bond
2,897 Insert is a very small tag - confused about the protocol? by Cate	1	9,756	2014-12-10 16:42:43 by Steve Bond
2,898 More than 3 fragments by dcunha	1	7,288	2014-12-08 21:44:34 by Steve Bond
2,899 empty vector by vithya	1	7,822	2014-10-23 18:45:23 by Steve Bond
2,900 Question on the basics by Sand	3	13,461	2014-08-11 20:08:05 by Steve Bond
2,901 Cloningtroubles when 'swapping' genes by Meyana	6	20,387	2014-08-05 11:02:03 by Steve Bond
2,902 Removing part by Sand	3	11,987	2014-07-26 15:03:44 by Steve Bond
2,903 Length of plasmid by Jan	5	19,316	2014-07-25 13:54:12 by Steve Bond
2,904 Cloning not working! Any advice? by erin.green	5	19,423	2014-07-13 15:30:18 by Steve Bond
2,905 Always "empty" vectors...? by Tiago	4	24,640	2014-07-10 10:25:05 by Tiago
2,906 Suggestion for Primer design and conditions by Tamjeed	7	18,918	2014-06-30 19:55:21 by Danielbut
2,907 Any suggestions? by galcafri	9	26,286	2014-06-04 22:52:45 by Steve Bond
2,908 any ideas on what I am doing wrong? by ebeisn2	4	18,234	2014-02-07 22:59:45 by Steve Bond

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