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rf-cloning troubles

Topic	Replies	Views	Last post
2,611 First PCR went OK, second PCR does not work. by Sybr_Green	0	8,079	2023-03-03 17:39:23 by Sybr_Green
2,612 Trouble for primary PCR by Shubhangivirkar	0	1,187	2022-11-15 02:29:52 by Shubhangivirkar
2,613 Small insertion into big plasmid, can't get any colony by Sybr_Green	3	3,901	2022-01-21 00:32:03 by Steve Bond
2,614 RF cloning first PCR by Nassiima	3	4,203	2021-06-22 13:02:20 by Nassiima
2,615 trouble with 9 kbp plasmid by copperD	2	8,117	2021-05-31 12:23:32 by alexsunny123
2,616 Unspecific binding of primers to target by rfuser	1	3,762	2021-03-06 16:23:22 by Steve Bond
2,617 Secondary PCR transformation not working by janhavi23	4	5,312	2021-02-14 16:51:47 by Steve Bond
2,618 No positive colony after 2nd PCR,is high T-A concentration a problems? by linh_	2	4,279	2021-01-18 00:36:19 by linh_
2,619 Second PCR not working by Manu	4	5,868	2021-01-15 07:29:32 by linh_
2,620 Ordered primers that make the entire insert - overlap on both ends by jk1234	1	3,244	2020-08-30 09:08:34 by Steve Bond
2,621 Using electrocompetent cells by kjkjindal	1	4,000	2020-07-14 09:11:06 by Steve Bond
2,622 Cant get any colonies by asli84	1	4,146	2020-03-03 02:06:04 by Steve Bond
2,623 Issues with the Secondary PCR reaction by koikkarah93	1	4,773	2019-08-27 00:16:59 by Steve Bond
2,624 trouble with 2nd PCR! Please help by hyla	4	8,755	2019-06-23 12:41:59 by Steve Bond
2,625 Colony PCR for small inserts by Ranjani	3	8,763	2018-08-11 14:32:29 by Steve Bond
2,626 Fake colony by jailanian16	11	22,176	2018-05-31 15:21:04 by Steve Bond
2,627 Any data analyzing tool suggestion? by bioscience	2	7,503	2018-04-13 16:47:03 by Steve Bond
2,628 2nd PCR not working by ellenmcmullen	1	6,133	2018-03-11 13:13:42 by Steve Bond
2,629 Secondary PCR trouble by abhinit	3	9,362	2017-12-19 15:30:38 by abhinit
2,630 RF cloning secondary PCR troubles. by Abhinit	1	3,983	2017-12-18 15:20:36 by NagarA
2,631 Problem with Secondary PCR by gunception	7	21,092	2017-12-02 15:45:32 by Steve Bond
2,632 DpnI: Heat Inactivation Necessary or Detrimental? by ChrisC	1	12,054	2017-08-29 12:38:13 by Steve Bond
2,633 only original (un-edited) plasmids grow by snissim	1	7,120	2017-07-18 22:04:34 by Steve Bond
2,634 Insert size by cblank	3	11,424	2017-06-23 12:59:45 by Steve Bond
2,635 Secondary PCR not working by asilbering	5	15,127	2017-02-28 14:45:56 by asilbering
2,636 Rf Cloning false positives by filipebaeta	1	6,823	2017-01-23 15:49:44 by Steve Bond
2,637 How to get insert:vector ratio in the 2' PCR? by minyoungfan33	1	7,265	2016-10-05 10:55:01 by Steve Bond
2,638 Relevance of DpnI treatment? by Alexander	4	23,317	2016-05-09 12:37:42 by CharlesPt
2,639 Amount of mega primer/plasmids second PCR. by Jan	5	16,993	2016-05-09 12:36:35 by CharlesPt
2,640 Problem with RF cloning by shrikant	1	7,247	2016-05-01 14:06:31 by Steve Bond

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