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rf-cloning troubles

Topic	Replies	Views	Last post
1,561 First PCR went OK, second PCR does not work. by Sybr_Green	0	8,194	2023-03-03 17:39:23 by Sybr_Green
1,562 Trouble for primary PCR by Shubhangivirkar	0	1,288	2022-11-15 02:29:52 by Shubhangivirkar
1,563 Small insertion into big plasmid, can't get any colony by Sybr_Green	3	4,151	2022-01-21 00:32:03 by Steve Bond
1,564 RF cloning first PCR by Nassiima	3	4,448	2021-06-22 13:02:20 by Nassiima
1,565 trouble with 9 kbp plasmid by copperD	2	8,302	2021-05-31 12:23:32 by alexsunny123
1,566 Unspecific binding of primers to target by rfuser	1	3,937	2021-03-06 16:23:22 by Steve Bond
1,567 Secondary PCR transformation not working by janhavi23	4	5,582	2021-02-14 16:51:47 by Steve Bond
1,568 No positive colony after 2nd PCR,is high T-A concentration a problems? by linh_	2	4,518	2021-01-18 00:36:19 by linh_
1,569 Second PCR not working by Manu	4	6,174	2021-01-15 07:29:32 by linh_
1,570 Ordered primers that make the entire insert - overlap on both ends by jk1234	1	3,407	2020-08-30 09:08:34 by Steve Bond
1,571 Using electrocompetent cells by kjkjindal	1	4,163	2020-07-14 09:11:06 by Steve Bond
1,572 Cant get any colonies by asli84	1	4,310	2020-03-03 02:06:04 by Steve Bond
1,573 Issues with the Secondary PCR reaction by koikkarah93	1	4,931	2019-08-27 00:16:59 by Steve Bond
1,574 trouble with 2nd PCR! Please help by hyla	4	9,032	2019-06-23 12:41:59 by Steve Bond
1,575 Colony PCR for small inserts by Ranjani	3	9,019	2018-08-11 14:32:29 by Steve Bond
1,576 Fake colony by jailanian16	11	22,860	2018-05-31 15:21:04 by Steve Bond
1,577 Any data analyzing tool suggestion? by bioscience	2	7,644	2018-04-13 16:47:03 by Steve Bond
1,578 2nd PCR not working by ellenmcmullen	1	6,276	2018-03-11 13:13:42 by Steve Bond
1,579 Secondary PCR trouble by abhinit	3	9,630	2017-12-19 15:30:38 by abhinit
1,580 RF cloning secondary PCR troubles. by Abhinit	1	4,091	2017-12-18 15:20:36 by NagarA
1,581 Problem with Secondary PCR by gunception	7	21,548	2017-12-02 15:45:32 by Steve Bond
1,582 DpnI: Heat Inactivation Necessary or Detrimental? by ChrisC	1	12,274	2017-08-29 12:38:13 by Steve Bond
1,583 only original (un-edited) plasmids grow by snissim	1	7,283	2017-07-18 22:04:34 by Steve Bond
1,584 Insert size by cblank	3	11,685	2017-06-23 12:59:45 by Steve Bond
1,585 Secondary PCR not working by asilbering	5	15,446	2017-02-28 14:45:56 by asilbering
1,586 Rf Cloning false positives by filipebaeta	1	6,988	2017-01-23 15:49:44 by Steve Bond
1,587 How to get insert:vector ratio in the 2' PCR? by minyoungfan33	1	7,428	2016-10-05 10:55:01 by Steve Bond
1,588 Relevance of DpnI treatment? by Alexander	4	23,617	2016-05-09 12:37:42 by CharlesPt
1,589 Amount of mega primer/plasmids second PCR. by Jan	5	17,299	2016-05-09 12:36:35 by CharlesPt
1,590 Problem with RF cloning by shrikant	1	7,443	2016-05-01 14:06:31 by Steve Bond

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