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rf-cloning troubles

Topic	Replies	Views	Last post
1,021 First PCR went OK, second PCR does not work. by Sybr_Green	0	8,163	2023-03-03 17:39:23 by Sybr_Green
1,022 Trouble for primary PCR by Shubhangivirkar	0	1,262	2022-11-15 02:29:52 by Shubhangivirkar
1,023 Small insertion into big plasmid, can't get any colony by Sybr_Green	3	4,080	2022-01-21 00:32:03 by Steve Bond
1,024 RF cloning first PCR by Nassiima	3	4,382	2021-06-22 13:02:20 by Nassiima
1,025 trouble with 9 kbp plasmid by copperD	2	8,266	2021-05-31 12:23:32 by alexsunny123
1,026 Unspecific binding of primers to target by rfuser	1	3,888	2021-03-06 16:23:22 by Steve Bond
1,027 Secondary PCR transformation not working by janhavi23	4	5,514	2021-02-14 16:51:47 by Steve Bond
1,028 No positive colony after 2nd PCR,is high T-A concentration a problems? by linh_	2	4,450	2021-01-18 00:36:19 by linh_
1,029 Second PCR not working by Manu	4	6,099	2021-01-15 07:29:32 by linh_
1,030 Ordered primers that make the entire insert - overlap on both ends by jk1234	1	3,369	2020-08-30 09:08:34 by Steve Bond
1,031 Using electrocompetent cells by kjkjindal	1	4,128	2020-07-14 09:11:06 by Steve Bond
1,032 Cant get any colonies by asli84	1	4,273	2020-03-03 02:06:04 by Steve Bond
1,033 Issues with the Secondary PCR reaction by koikkarah93	1	4,894	2019-08-27 00:16:59 by Steve Bond
1,034 trouble with 2nd PCR! Please help by hyla	4	8,970	2019-06-23 12:41:59 by Steve Bond
1,035 Colony PCR for small inserts by Ranjani	3	8,952	2018-08-11 14:32:29 by Steve Bond
1,036 Fake colony by jailanian16	11	22,693	2018-05-31 15:21:04 by Steve Bond
1,037 Any data analyzing tool suggestion? by bioscience	2	7,609	2018-04-13 16:47:03 by Steve Bond
1,038 2nd PCR not working by ellenmcmullen	1	6,246	2018-03-11 13:13:42 by Steve Bond
1,039 Secondary PCR trouble by abhinit	3	9,560	2017-12-19 15:30:38 by abhinit
1,040 RF cloning secondary PCR troubles. by Abhinit	1	4,060	2017-12-18 15:20:36 by NagarA
1,041 Problem with Secondary PCR by gunception	7	21,432	2017-12-02 15:45:32 by Steve Bond
1,042 DpnI: Heat Inactivation Necessary or Detrimental? by ChrisC	1	12,210	2017-08-29 12:38:13 by Steve Bond
1,043 only original (un-edited) plasmids grow by snissim	1	7,244	2017-07-18 22:04:34 by Steve Bond
1,044 Insert size by cblank	3	11,626	2017-06-23 12:59:45 by Steve Bond
1,045 Secondary PCR not working by asilbering	5	15,390	2017-02-28 14:45:56 by asilbering
1,046 Rf Cloning false positives by filipebaeta	1	6,943	2017-01-23 15:49:44 by Steve Bond
1,047 How to get insert:vector ratio in the 2' PCR? by minyoungfan33	1	7,381	2016-10-05 10:55:01 by Steve Bond
1,048 Relevance of DpnI treatment? by Alexander	4	23,549	2016-05-09 12:37:42 by CharlesPt
1,049 Amount of mega primer/plasmids second PCR. by Jan	5	17,218	2016-05-09 12:36:35 by CharlesPt
1,050 Problem with RF cloning by shrikant	1	7,391	2016-05-01 14:06:31 by Steve Bond

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