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rf-cloning troubles

Topic	Replies	Views	Last post
3,991 Insert size by cblank	3	12,018	2017-06-23 12:59:45 by Steve Bond
3,992 Secondary PCR not working by asilbering	5	15,857	2017-02-28 14:45:56 by asilbering
3,993 Rf Cloning false positives by filipebaeta	1	7,202	2017-01-23 15:49:44 by Steve Bond
3,994 How to get insert:vector ratio in the 2' PCR? by minyoungfan33	1	7,612	2016-10-05 10:55:01 by Steve Bond
3,995 Relevance of DpnI treatment? by Alexander	4	23,981	2016-05-09 12:37:42 by CharlesPt
3,996 Amount of mega primer/plasmids second PCR. by Jan	5	17,734	2016-05-09 12:36:35 by CharlesPt
3,997 Problem with RF cloning by shrikant	1	7,688	2016-05-01 14:06:31 by Steve Bond
3,998 No colonies & no band on agarose gel after 2°PCR by afahrny	3	13,681	2015-12-21 11:24:24 by Steve Bond
3,999 to design an RF cloning project with the website by mandyscott	0	9,547	2015-12-21 06:00:15 by mandyscott
4,000 No band for products at all after primary PCR by bubunmicro@gmail.com	7	28,984	2015-06-30 20:14:40 by Steve Bond
4,001 suggestions for primary PCR by vithya	5	18,680	2015-05-10 14:22:04 by Steve Bond
4,002 RF-cloning to do mutagenesis? by erin.green	6	18,896	2015-02-18 17:16:07 by Steve Bond
4,003 Procedure after DpnI digestion by hongrulin	4	17,671	2015-02-18 17:15:33 by Steve Bond
4,004 DpnI by Sand	1	8,195	2015-01-30 16:15:24 by Steve Bond
4,005 Is phosphorilation needed? by Dungeonlord	18	57,608	2015-01-24 00:10:26 by Steve Bond
4,006 2nd PCR not working by ZacJ	8	81,565	2014-12-16 16:58:59 by ayidazagu
4,007 RFP + gene of interest RF cloning into vector in place of GFP ? by vsustar	2	31,484	2014-12-14 23:22:26 by LucilleHib
4,008 Problematic to say the least by pcr	7	26,696	2014-12-10 22:51:54 by Steve Bond
4,009 Insert is a very small tag - confused about the protocol? by Cate	1	9,862	2014-12-10 16:42:43 by Steve Bond
4,010 More than 3 fragments by dcunha	1	7,397	2014-12-08 21:44:34 by Steve Bond
4,011 empty vector by vithya	1	7,927	2014-10-23 18:45:23 by Steve Bond
4,012 Question on the basics by Sand	3	13,622	2014-08-11 20:08:05 by Steve Bond
4,013 Cloningtroubles when 'swapping' genes by Meyana	6	20,626	2014-08-05 11:02:03 by Steve Bond
4,014 Removing part by Sand	3	12,141	2014-07-26 15:03:44 by Steve Bond
4,015 Length of plasmid by Jan	5	19,592	2014-07-25 13:54:12 by Steve Bond
4,016 Cloning not working! Any advice? by erin.green	5	19,706	2014-07-13 15:30:18 by Steve Bond
4,017 Always "empty" vectors...? by Tiago	4	24,866	2014-07-10 10:25:05 by Tiago
4,018 Suggestion for Primer design and conditions by Tamjeed	7	19,175	2014-06-30 19:55:21 by Danielbut
4,019 Any suggestions? by galcafri	9	26,598	2014-06-04 22:52:45 by Steve Bond
4,020 any ideas on what I am doing wrong? by ebeisn2	4	18,401	2014-02-07 22:59:45 by Steve Bond

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