User note

May 22nd: I've just received some very interesting feedback from a colleague, who found that switching to a two-step protocol of 72° and 98° for the secondary PCR reaction dramatically increases the efficiency of his projects. In other words, ignore the calculated annealing temperature and go straight to the extension step. If anyone out there has been struggling with super low efficiency, please give that a try and shoot me an email to let me know if it works better for you. If I get some more positive feedback, I'll make changes to the website.
-Steve

Backbone name:
Insert name:
Insert description:
Orientation: (+) (-)
Arrow: On Off
Advanced settings

Plasmid Sequence Specify Insert Sites: 1 2
Or, place 2 exclaimation points (!) directly in the sequence to denote where you want the insert to go.






Insert Sequence











© Steve Bond 2009-13
Privacy statement